At what point in their conversation does Hamlet realize Rosencrantz and Guildenstern are spies, and how does he indirectly tell them he no longer trusts them?

At what point in their conversation does Hamlet realize Rosencrantz and Guildenstern are spies, and how does he indirectly tell them he no longer trusts them?
Hamlet accuses the two of being sent by Claudius and Gertrude because he can see through the deception of Rosencrantz and Guildenstern.

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